Bone Sialoprotein Polyclonal Antibody, FITC Conjugated

MG63 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bone Sialoprotein 2 Antibody,FITC Conjugated(bs-4729R-FITC)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature.Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).