NADPH oxidase 4 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4730R-Cy5.5 |
| Product Name | NADPH oxidase 4 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NOX4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 50507 |
| Swiss Prot | Q9NPH5 |
| Subcellular location | Cell membrane |
| Synonyms | Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX 1; KOX; Kox-1; NADPH; NADPH oxidase 4; Nox4; NOX4_HUMAN; Renal NADPH-oxidase; RENOX. |
| Background | Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is NADPH oxidase 4 Polyclonal Antibody, Cy5.5 Conjugated (BS-4730R-CY5-5) validated for, and at what dilution?
BS-4730R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NADPH oxidase 4 Polyclonal Antibody, Cy5.5 Conjugated (BS-4730R-CY5-5) react with?
Tested reactivity: Human.
How should BS-4730R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NADPH oxidase 4 Polyclonal Antibody, Cy5.5 Conjugated (BS-4730R-CY5-5)?
KLH conjugated synthetic peptide derived from human NOX4
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4730R-CY5-5)?
Cy5.5 on BS-4730R-CY5-5: excitation / emission 675nm/694nm.