CD19 Polyclonal Antibody

$Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD19 Polyclonal Antibody, Unconjugated(bs-4755R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD19 Polyclonal Antibody, Unconjugated(bs-4755R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution for 40 minutes at 37\u00b0C.

$Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated (bs-4755R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n$

Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated (bs-4755R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n

$Human HL60 Cells were fixed with 70% methanol (Overnight at 4\u2103). The cells were then incubated in 1 X PBS\/2%BSA\/10% goat serum to block non-specific protein-protein interactions followed by incubation with CD19 Polyclonal Antibody, Unconjugated (bs-4755R) at 0.2 \u03bcg\/10^6 cells for 30 minutes at room temp. A PE-conjugated secondary antibody was used for 40 min at room temperature. The graph compares the primary antibody (green) to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).$

Human HL60 Cells were fixed with 70% methanol (Overnight at 4\u2103). The cells were then incubated in 1 X PBS\/2%BSA\/10% goat serum to block non-specific protein-protein interactions followed by incubation with CD19 Polyclonal Antibody, Unconjugated (bs-4755R) at 0.2 \u03bcg\/10^6 cells for 30 minutes at room temp. A PE-conjugated secondary antibody was used for 40 min at room temperature. The graph compares the primary antibody (green) to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).

Applications

WB
FCM
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-4755R
Product Name	CD19 Polyclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD19
Immunogen Range	171-220/556
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID	930
Swiss Prot	P15391
Subcellular location	Extracellular
Synonyms	B4; CVID3; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19
Background	Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Application Dilution
WB	1:300-1000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

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