CD133 Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4770R-PerCP |
| Product Name | CD133 Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD133 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8842 |
| Swiss Prot | O43490 |
| Subcellular location | Cell membrane |
| Synonyms | AC133; Antigen AC133; Hematopoietic stem cell antigen; hProminin; PROM1; Prominin I; Prominin 1; Prominin1; Prominin-1; Prominin like protein 1 precursor; Prominin mouse like 1; prominin1; PROML1; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4. |
| Background | May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is CD133 Polyclonal Antibody, PerCP Conjugated (bs-4770R-PerCP) validated for, and at what dilution?
bs-4770R-PerCP is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD133 Polyclonal Antibody, PerCP Conjugated (bs-4770R-PerCP) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-4770R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD133 Polyclonal Antibody, PerCP Conjugated (bs-4770R-PerCP)?
KLH conjugated synthetic peptide derived from human CD133
What are the excitation and emission wavelengths of the PerCP conjugate (bs-4770R-PerCP)?
PerCP on bs-4770R-PerCP: excitation / emission 482nm/677nm.