HSPB2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4782R-Cy5.5-100uL |
| Product Name | HSPB2 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HSPB2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3316 |
| Swiss Prot | Q16082 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MKBP; HSP27; Hs.78846; LOH11CR1K; Heat shock protein beta-2; HspB2; DMPK-binding protein |
| Background | The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. HSPB2 (heat shock 27 kDa protein 2), also known as HSP 27 or MKBP, is a 182 amino acid protein that belongs to the heat shock protein family and is expressed preferentially in heart and skeletal muscle. Localized to mitochondria, HSPB2 functions as an ATP-dependent chaperone protein that plays a role in the refolding of denatured proteins and may also interact with the Actin cytoskeleton and prevent apoptotic cell death. HSPB2 is abundantly expressed in several cancer cell lines, suggesting that HSPB2 may be an important factor in tumor transformation and metastasis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HSPB2 Polyclonal Antibody, Cy5.5 Conjugated (BS-4782R-CY5-5) validated for, and at what dilution?
BS-4782R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSPB2 Polyclonal Antibody, Cy5.5 Conjugated (BS-4782R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Rabbit.
How should BS-4782R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSPB2 Polyclonal Antibody, Cy5.5 Conjugated (BS-4782R-CY5-5)?
KLH conjugated synthetic peptide derived from human HSPB2
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4782R-CY5-5)?
Cy5.5 on BS-4782R-CY5-5: excitation / emission 675nm/694nm.