CD45 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4819R-APC-Cy7-100uL |
| Product Name | CD45 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat CD45 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 24699 |
| Swiss Prot | P04157 |
| Subcellular location | Cell membrane |
| Synonyms | Lca; RT7; CD45; L-CA; T200; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; Ptprc |
| Background | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
Frequently asked questions
Which applications is CD45 Polyclonal Antibody, APC-Cy7 Conjugated (bs-4819R-APC-Cy7) validated for, and at what dilution?
bs-4819R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD45 Polyclonal Antibody, APC-Cy7 Conjugated (bs-4819R-APC-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-4819R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD45 Polyclonal Antibody, APC-Cy7 Conjugated (bs-4819R-APC-Cy7)?
KLH conjugated synthetic peptide derived from rat CD45
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-4819R-APC-Cy7)?
APC-Cy7 on bs-4819R-APC-Cy7: excitation / emission 650nm/780nm.