LC3B Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-4843R-100uL |
| Product Name | LC3B Polyclonal Antibody |
| Applications |
WB
FCM
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Microtubule-associated proteins 1A/1B light chain 3B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Synonyms | Microtubule-associated protein 1 light chain 3 beta;ATG8F; Autophagy related protein LC3 B; Autophagy related ubiquitin like modier LC3 B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modier LC3 B; MAP1 light chain 3 like protein 2; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1ALC3; MAP1LC3B; Microtubule associated protein 1 light chain 3 beta; Microtubule associated proteins 1A/1B light chain 3B; Microtubule-associated protein 1 light chain 3 beta; Microtubule-associated proteins 1A/1B light chain 3B; MLP3B_HUMAN. |
| Background | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3 Beta) and MAP-light chain 3 alpha (MAP-LC3 Alpha) are subunits of both MAP1A and MAP1B. MAP-LC3 Beta, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3 Beta, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3 Beta, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3 Beta may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | 1:100 |
| IHC-P | 1:100-500 |
Frequently asked questions
Which applications is LC3B Polyclonal Antibody (bs-4843R) validated for, and at what dilution?
bs-4843R is validated for WB (1:500-2000); FCM (1:100); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LC3B Polyclonal Antibody (bs-4843R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-4843R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LC3B Polyclonal Antibody (bs-4843R)?
KLH conjugated synthetic peptide derived from human Microtubule-associated proteins 1A/1B light chain 3B