Factor VII heavy chain Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4846R-BF750-100uL |
| Product Name | Factor VII heavy chain Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Factor VII heavy chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2155 |
| Swiss Prot | P08709 |
| Subcellular location | Secreted |
| Synonyms | SPCA; Coagulation factor VII; Proconvertin; Serum prothrombin conversion accelerator; F7 |
| Background | Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Factor VII heavy chain Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-4846R-BF750) validated for, and at what dilution?
bs-4846R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Factor VII heavy chain Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-4846R-BF750) react with?
Tested reactivity: Human.
How should bs-4846R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Factor VII heavy chain Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-4846R-BF750)?
KLH conjugated synthetic peptide derived from human Factor VII heavy chain
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-4846R-BF750)?
AbBy Fluor-750 on bs-4846R-BF750: excitation / emission 749nm/775nm.