Factor VII heavy chain Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4846R-Cy5.5-100uL |
| Product Name | Factor VII heavy chain Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Factor VII heavy chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2155 |
| Swiss Prot | P08709 |
| Subcellular location | Secreted |
| Synonyms | SPCA; Coagulation factor VII; Proconvertin; Serum prothrombin conversion accelerator; F7 |
| Background | Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Factor VII heavy chain Polyclonal Antibody, Cy5.5 Conjugated (BS-4846R-CY5-5) validated for, and at what dilution?
BS-4846R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Factor VII heavy chain Polyclonal Antibody, Cy5.5 Conjugated (BS-4846R-CY5-5) react with?
Tested reactivity: Human.
How should BS-4846R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Factor VII heavy chain Polyclonal Antibody, Cy5.5 Conjugated (BS-4846R-CY5-5)?
KLH conjugated synthetic peptide derived from human Factor VII heavy chain
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4846R-CY5-5)?
Cy5.5 on BS-4846R-CY5-5: excitation / emission 675nm/694nm.