phospho-RUNX3 (Ser338) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-4848R-APC-Cy7-100uL |
| Product Name | phospho-RUNX3 (Ser338) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human RUNX3 around the phosphorylation site of Ser338 |
| Modification Site | Ser338 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 864 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | p-RUNX3Ser338; RUNX 3; RUNX-3; Runt-related transcription factor 3; Core-binding factor, alpha 3 subunit; CBF-alpha 3; Acute myeloid leukemia 2 protein; OncogeneAML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEBP2-alpha C; PEA2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; Core-binding factor, alpha 3 subunit; CBF-alpha 3; Acute myeloid leukemia 2 protein; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEBP2-alpha C; RUNX3_HUMAN; PEA2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit. |
| Background | RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain.DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1, Subcellular location in Nucleus. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-RUNX3 (Ser338) Polyclonal Antibody, APC-Cy7 Conjugated (bs-4848R-APC-Cy7) validated for, and at what dilution?
bs-4848R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-4848R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-RUNX3 (Ser338) Polyclonal Antibody, APC-Cy7 Conjugated (bs-4848R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human RUNX3 around the phosphorylation site of Ser338
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-4848R-APC-Cy7)?
APC-Cy7 on bs-4848R-APC-Cy7: excitation / emission 650nm/780nm.