HCV NS4B Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4857R-Cy3 |
| Product Name | HCV NS4B Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Hepatitis C Virus NS4B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P26663 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | HCV; HCV NS4B; Non structural protein 4B; NS4B; p27. |
| Background | HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. NS4B induces a specific membrane alteration that serves as a scaffold for the virus replication complex. This membrane alteration gives rise to the so-called ER-derived membranous web that contains the replication complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HCV NS4B Polyclonal Antibody, Cy3 Conjugated (bs-4857R-Cy3) validated for, and at what dilution?
bs-4857R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HCV NS4B Polyclonal Antibody, Cy3 Conjugated (bs-4857R-Cy3) react with?
Tested reactivity: Virus.
How should bs-4857R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HCV NS4B Polyclonal Antibody, Cy3 Conjugated (bs-4857R-Cy3)?
KLH conjugated synthetic peptide derived from Hepatitis C Virus NS4B
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4857R-Cy3)?
Cy3 on bs-4857R-Cy3: excitation / emission 512,550nm/570,615nm.