Hepatitis C Virus RNA-directed RNA polymerase Antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Virus
Overview
Catalog # bs-4858R
Product Name Hepatitis C Virus RNA-directed RNA polymerase Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Virus
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from Hepatitis C Virus RNA-directed RNA polymerase
Immunogen Range 2501-2600/3010
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location Cytoplasm, Cell membrane
Synonyms RNA-directed RNA polymerase; p68; RNA dependent RNA polymerase.
Background The RNA directed RNA polymerase is also known as non-structural protein NS5B. NS5B is a 65 kDa protein that resembles other viral RNA polymerases. Hepatitis C virus (HCV) replication is thought to occur in membrane bound replication complexes. These complexes transcribe the positive strand and the resulting minus strand is used as a template for the synthesis of genomic RNA. There are two viral proteins involved in the reaction, NS3 and NS5B
Application Dilution
WB 1:500-2000
ELISA 1:5000-10000
IHC-P 1:100-500
IHC-F 1:100-500
IF(IHC-P) 1:100-500
IF(IHC-F) 1:100-500
IF(ICC) 1:100-500