Complement C3 beta chain Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4871R-PE-Cy5-100uL |
| Product Name | Complement C3 beta chain Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Specificity | This antibody could recognize C3-beta-c |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Complement C3 beta chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 718 |
| Swiss Prot | P01024 |
| Subcellular location | Secreted |
| Synonyms | ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p; Complement C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3; CO3_HUMAN |
| Background | C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:8376604, PubMed:2909530, PubMed:9059512, PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Complement C3 beta chain Antibody, PE-Cy5 Conjugated (bs-4871R-PE-Cy5) validated for, and at what dilution?
bs-4871R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Complement C3 beta chain Antibody, PE-Cy5 Conjugated (bs-4871R-PE-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-4871R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Complement C3 beta chain Antibody, PE-Cy5 Conjugated (bs-4871R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human Complement C3 beta chain
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-4871R-PE-Cy5)?
PE-Cy5 on bs-4871R-PE-Cy5: excitation / emission 488nm/670nm.