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Complement C3c alpha' chain fragment 1 Antibody, AbBy Fluor-405 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
Overview
Catalog # bs-4874r-bf405
Product Name Complement C3c alpha' chain fragment 1 Antibody, AbBy Fluor-405 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Complement C3c alpha' chain fragment 1
Immunogen Range 749-850/1663
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 718
Swiss Prot p01024
Subcellular location Secreted
Synonyms Acylation stimulating protein; ASP; C3; C3a anaphylatoxin; Complement C3 alpha chain; Complement C3; Complement C3 precursor; Complement component 3; Complement component C3; Complement factor 3; Plp; Complement C3c alpha' chain fragment 1; CO3_HUMAN.
Background The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Complement C3c alpha' chain fragment 1 Antibody, AbBy Fluor-405 Conjugated (bs-4874R-BF405) validated for, and at what dilution?

bs-4874R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-4874R-BF405 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Complement C3c alpha' chain fragment 1 Antibody, AbBy Fluor-405 Conjugated (bs-4874R-BF405)?

KLH conjugated synthetic peptide derived from human Complement C3c alpha' chain fragment 1 Immunogen range: 749-850/1663.

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-4874R-BF405)?

AbBy Fluor-405 on bs-4874R-BF405: excitation / emission 401nm/421nm.