Complement C3c alpha' chain fragment 1 Antibody, PE Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-4874r-pe |
| Product Name | Complement C3c alpha' chain fragment 1 Antibody, PE Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Complement C3c alpha' chain fragment 1 |
| Immunogen Range | 749-850/1663 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 718 |
| Swiss Prot | p01024 |
| Subcellular location | Secreted |
| Synonyms | Acylation stimulating protein; ASP; C3; C3a anaphylatoxin; Complement C3 alpha chain; Complement C3; Complement C3 precursor; Complement component 3; Complement component C3; Complement factor 3; Plp; Complement C3c alpha' chain fragment 1; CO3_HUMAN. |
| Background | The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Complement C3c alpha' chain fragment 1 Antibody, PE Conjugated (bs-4874R-PE) validated for, and at what dilution?
bs-4874R-PE is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-4874R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Complement C3c alpha' chain fragment 1 Antibody, PE Conjugated (bs-4874R-PE)?
KLH conjugated synthetic peptide derived from human Complement C3c alpha' chain fragment 1 Immunogen range: 749-850/1663.
What are the excitation and emission wavelengths of the PE conjugate (bs-4874R-PE)?
PE on bs-4874R-PE: excitation / emission 496,564nm/578nm.