CITED1 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4898R-PE-Cy5 |
| Product Name | CITED1 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Specificity | May cross react with CITED2 |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CITED1/ABCC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10370 |
| Swiss Prot | Q99967 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ASD8; MRG1; VSD2; MRG-1; P35SRJ; Cbp/p300-interacting transactivator 2; MSG-related protein 1; CITED2 |
| Background | Transcriptional coactivator of the p300/CBP-mediated trancription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator-activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Acts also as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left-right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CITED1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-4898R-PE-Cy5) validated for, and at what dilution?
bs-4898R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CITED1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-4898R-PE-Cy5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig.
How should bs-4898R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CITED1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-4898R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human CITED1/ABCC1
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-4898R-PE-Cy5)?
PE-Cy5 on bs-4898R-PE-Cy5: excitation / emission 488nm/670nm.