Latency-associated peptide Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4908R-Cy5.5-100uL |
| Product Name | Latency-associated peptide Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | The antibody targets the Latency-associated peptide (LAP), which is a cleavage product of the Transforming growth factor beta-1 proprotein (TGFB1) |
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TGF beta 1/LAP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7040 |
| Swiss Prot | P01137 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | Transforming growth factor beta-1 proprotein; CED; LAP; DPD1; TGFB; TGFbeta; TGF-beta-1; TGFB1 |
| Background | Latency-associated peptide: Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia (Probable). Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Latency-associated peptide Polyclonal Antibody, Cy5.5 Conjugated (BS-4908R-CY5-5) validated for, and at what dilution?
BS-4908R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Latency-associated peptide Polyclonal Antibody, Cy5.5 Conjugated (BS-4908R-CY5-5) react with?
Tested reactivity: Human.
How should BS-4908R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Latency-associated peptide Polyclonal Antibody, Cy5.5 Conjugated (BS-4908R-CY5-5)?
KLH conjugated synthetic peptide derived from human TGF beta 1/LAP
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4908R-CY5-5)?
Cy5.5 on BS-4908R-CY5-5: excitation / emission 675nm/694nm.