ENPP1 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4913R-APC-100uL |
| Product Name | ENPP1 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ENPP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5167 |
| Swiss Prot | P22413 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Membrane component chromosome 6 surface marker 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1; ENPP1; PC1 |
| Background | By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity and function. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ENPP1 Polyclonal Antibody, APC Conjugated (bs-4913R-APC) validated for, and at what dilution?
bs-4913R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ENPP1 Polyclonal Antibody, APC Conjugated (bs-4913R-APC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Pig, Horse, Rabbit.
How should bs-4913R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ENPP1 Polyclonal Antibody, APC Conjugated (bs-4913R-APC)?
KLH conjugated synthetic peptide derived from human ENPP1
What are the excitation and emission wavelengths of the APC conjugate (bs-4913R-APC)?
APC on bs-4913R-APC: excitation / emission 650nm/660nm.