EAAT2 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-49183r-rbitc |
| Product Name | EAAT2 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | Recombinant mouse EAAT2 protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6506 |
| Swiss Prot | P43006 |
| Subcellular location | Cell membrane |
| Synonyms | EAAT2; Excitatory amino acid transporter 2; Excitotoxic amino acid transporter 2; Glial high affinity glutamate transporter; GLT 1; GLT1; Glutamate aspartate transporter II; SLC1A2; Sodium dependent glutamate aspartate transporter 2; Solute carrier family 1 glial high affinity glutamate transporter member 2; Solute carrier family 1 member 2; Excitatory amino acid transporter 2; GLT-1; SLC1A2; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2. |
| Background | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate. |
| Application Dilution | |
| WB | =1:500-2000 |
| IF | IF=1:100-500 |
Frequently asked questions
Which applications is EAAT2 Polyclonal Antibody, RBITC Conjugated (bs-49183R-RBITC) validated for, and at what dilution?
bs-49183R-RBITC is validated for WB (WB=1:500-2000); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EAAT2 Polyclonal Antibody, RBITC Conjugated (bs-49183R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-49183R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EAAT2 Polyclonal Antibody, RBITC Conjugated (bs-49183R-RBITC)?
Recombinant mouse EAAT2 protein
What are the excitation and emission wavelengths of the RBITC conjugate (bs-49183R-RBITC)?
RBITC on bs-49183R-RBITC: excitation / emission 570nm/595nm.