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hIgG4 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bs-49189R-Cy3
Product Name hIgG4 Polyclonal Antibody, Cy3 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source hIgG4 protein
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Application Dilution
WB =1:500-2000

Frequently asked questions

Which applications is hIgG4 Polyclonal Antibody, Cy3 Conjugated (bs-49189R-Cy3) validated for, and at what dilution?

bs-49189R-Cy3 is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does hIgG4 Polyclonal Antibody, Cy3 Conjugated (bs-49189R-Cy3) react with?

Tested reactivity: Human.

How should bs-49189R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise hIgG4 Polyclonal Antibody, Cy3 Conjugated (bs-49189R-Cy3)?

hIgG4 protein

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-49189R-Cy3)?

Cy3 on bs-49189R-Cy3: excitation / emission 512,550nm/570,615nm.