MSH3 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4919R-100uL |
| Product Name | MSH3 Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MSH3 |
| Immunogen Range | 521-620/1137 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4437 |
| Swiss Prot | P20585 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DUP; MRP1; DNA mismatch repair protein Msh3; hMSH3; Divergent upstream protein; Mismatch repair protein 1; MSH3; DUC1; DUG |
| Background | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF | |
Frequently asked questions
Which applications is MSH3 Polyclonal Antibody (bs-4919R) validated for, and at what dilution?
bs-4919R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MSH3 Polyclonal Antibody (bs-4919R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Rabbit.
How should bs-4919R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MSH3 Polyclonal Antibody (bs-4919R)?
KLH conjugated synthetic peptide derived from human MSH3 Immunogen range: 521-620/1137.