HIV1 p55+p24+p17 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4942R-APC-Cy7-100uL |
| Product Name | HIV1 p55+p24+p17 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from HIV1 Pr55Gag |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | HIV p55+Capsid protein p24; HIV1 p55 + p24 + p17; CA antibody Capsid protein p24; Gag; Gag polyprotein; HIV1 matrix protein p17; HIV1 Pr55Gag; Human immunodeficiency virus 1 p17; Human immunodeficiency virus 1 p55; Human immunodeficiency virus type 1 p24; MA antibody Matrix protein; Matrix protein p17; Pr55; Pr55Gag; POL_HV1H2. |
| Background | HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HIV1 p55+p24+p17 Polyclonal Antibody, APC-Cy7 Conjugated (bs-4942R-APC-Cy7) validated for, and at what dilution?
bs-4942R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIV1 p55+p24+p17 Polyclonal Antibody, APC-Cy7 Conjugated (bs-4942R-APC-Cy7) react with?
Tested reactivity: Virus.
How should bs-4942R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIV1 p55+p24+p17 Polyclonal Antibody, APC-Cy7 Conjugated (bs-4942R-APC-Cy7)?
KLH conjugated synthetic peptide derived from HIV1 Pr55Gag
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-4942R-APC-Cy7)?
APC-Cy7 on bs-4942R-APC-Cy7: excitation / emission 650nm/780nm.