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MPO Polyclonal Antibody, PerCP Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-4943R-PerCP
Product Name MPO Polyclonal Antibody, PerCP Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Horse, Rabbit, GuineaPig
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MPO
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4353
Swiss Prot P05164
Subcellular location Cytoplasm
Synonyms Myeloperoxidase; MPO
Background Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MPO Polyclonal Antibody, PerCP Conjugated (bs-4943R-PerCP) validated for, and at what dilution?

bs-4943R-PerCP is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MPO Polyclonal Antibody, PerCP Conjugated (bs-4943R-PerCP) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Horse, Rabbit, GuineaPig.

How should bs-4943R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MPO Polyclonal Antibody, PerCP Conjugated (bs-4943R-PerCP)?

KLH conjugated synthetic peptide derived from human MPO

What are the excitation and emission wavelengths of the PerCP conjugate (bs-4943R-PerCP)?

PerCP on bs-4943R-PerCP: excitation / emission 482nm/677nm.