eIF4E Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4979R-BF405 |
| Product Name | eIF4E Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eIF4E |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1977 |
| Swiss Prot | P06730 |
| Subcellular location | Cytoplasm |
| Synonyms | CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein |
| Background | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is eIF4E Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-4979R-BF405) validated for, and at what dilution?
bs-4979R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF4E Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-4979R-BF405) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-4979R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF4E Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-4979R-BF405)?
KLH conjugated synthetic peptide derived from human eIF4E
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-4979R-BF405)?
AbBy Fluor-405 on bs-4979R-BF405: excitation / emission 401nm/421nm.