HIV p55+P6-Gag Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4980R-BF350-100uL |
| Product Name | HIV p55+P6-Gag Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Virus, Others |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from HIV P6-Gag |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | HIV1 Pr55Gag; Human immunodeficiency virus 1; Gag protein p6; MA antibody Matrix protein; Matrix protein p17; Pr55; Pr55Gag. |
| Background | Human immunodeficiency virus (HIV) is a retrovirus that can lead to a condition in which the immune system begins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the human immune system such as helper T cells(specifically CD4+ T cells), macrophages and dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosis in infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytes that recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genome is converted to double-stranded DNA by a virally encoded reverse transcriptase that is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integrase so that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latent and the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HIV p55+P6-Gag Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-4980R-BF350) validated for, and at what dilution?
bs-4980R-BF350 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIV p55+P6-Gag Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-4980R-BF350) react with?
Tested reactivity: Virus, Others.
How should bs-4980R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIV p55+P6-Gag Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-4980R-BF350)?
KLH conjugated synthetic peptide derived from HIV P6-Gag
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-4980R-BF350)?
AbBy Fluor-350 on bs-4980R-BF350: excitation / emission 346nm/442nm.