HIV p55+NP7 Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4981R-PerCP-100uL |
| Product Name | HIV p55+NP7 Polyclonal Antibody, PerCP Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will recognize the full Gag protein and Nucleocapsid protein p7 after cleavage |
| Reactivity | Virus |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from HIV1 Nucleocapsid protein p7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | HIV1 Pr55Gag; Human immunodeficiency virus 1; Gag protein; Nucleocapsid protein p7; |
| Background | HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HIV p55+NP7 Polyclonal Antibody, PerCP Conjugated (bs-4981R-PerCP) validated for, and at what dilution?
bs-4981R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIV p55+NP7 Polyclonal Antibody, PerCP Conjugated (bs-4981R-PerCP) react with?
Tested reactivity: Virus.
How should bs-4981R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIV p55+NP7 Polyclonal Antibody, PerCP Conjugated (bs-4981R-PerCP)?
KLH conjugated synthetic peptide derived from HIV1 Nucleocapsid protein p7
What are the excitation and emission wavelengths of the PerCP conjugate (bs-4981R-PerCP)?
PerCP on bs-4981R-PerCP: excitation / emission 482nm/677nm.