Rb2 p130 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-4984R-BF680-100uL |
| Product Name | Rb2 p130 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Rb2 p130 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | 130 kDa retinoblastoma associated protein; FLJ26459; p130; PRB 2; PRB2; Rb 2; Rb2; RBL2; RBR 2; RBR2; Retinoblastoma like 2; Retinoblastoma like protein 2; Retinoblastoma Related Gene. |
| Background | p130 is related both in structure and function to the retinoblastoma tumor suppressor protein and p107 (collectively known as pocket proteins) and is known to regulate the activity of E2F transcription factors. E2F transcription factors regulate the expression of a number of genes important in cell proliferation, particularly those involved in the progression through G1 and into the S phase of the cell cycle. Binding of p130 converts E2F transcription factors from transcriptional activators to transcriptional repressors. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Rb2 p130 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4984R-BF680) validated for, and at what dilution?
bs-4984R-BF680 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-4984R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Rb2 p130 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4984R-BF680)?
KLH conjugated synthetic peptide derived from human Rb2 p130
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-4984R-BF680)?
AbBy Fluor-680 on bs-4984R-BF680: excitation / emission 679nm/702nm.