PYGM Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5012R-Cy5.5-100uL |
| Product Name | PYGM Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PYGM |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5837 |
| Swiss Prot | P11217 |
| Subcellular location | Cytoplasm, Extracellular matrix |
| Synonyms | Glycogen phosphorylase, muscle form; Myophosphorylase; PYGM |
| Background | Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PYGM Polyclonal Antibody, Cy5.5 Conjugated (BS-5012R-CY5-5) validated for, and at what dilution?
BS-5012R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PYGM Polyclonal Antibody, Cy5.5 Conjugated (BS-5012R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse.
How should BS-5012R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PYGM Polyclonal Antibody, Cy5.5 Conjugated (BS-5012R-CY5-5)?
KLH conjugated synthetic peptide derived from human PYGM
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5012R-CY5-5)?
Cy5.5 on BS-5012R-CY5-5: excitation / emission 675nm/694nm.