AGPAT1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5023R-Cy5-100uL |
| Product Name | AGPAT1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AGPAT1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10554 |
| Subcellular location | Cytoplasm |
| Synonyms | 1 AGPAT1; LPAAT alpha; LPAATA; MGC5423; 1 acyl sn glycerol 3 phosphate acyltransferase alpha; 1 acylglycerol 3 phosphate O acyltransferase 1 acetoacetly Coenzyme A thiolase ; 1 acylglycerol 3 phosphate O acyltransferase 1 lysophosphatidic acid acyltransferase alpha; 1 acylglycerol 3 phosphate O acyltransferase 1; 1 AGP acyltransferase 1; AGPAT 1; G15 antibody Lysophosphatidic acid acyltransferase alpha; Lysophospholipid acyltransferase; MGC4007; OTTHUMP00000062693; Protein G15; Protein-G15. PLCA_HUMAN |
| Background | AGPAT1 converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AGPAT1 Polyclonal Antibody, Cy5 Conjugated (bs-5023R-Cy5) validated for, and at what dilution?
bs-5023R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AGPAT1 Polyclonal Antibody, Cy5 Conjugated (bs-5023R-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-5023R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AGPAT1 Polyclonal Antibody, Cy5 Conjugated (bs-5023R-Cy5)?
KLH conjugated synthetic peptide derived from human AGPAT1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5023R-Cy5)?
Cy5 on bs-5023R-Cy5: excitation / emission 625,650nm/670nm.