Angiopoietin-like 4 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5027R-Cy3 |
| Product Name | Angiopoietin-like 4 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse ARP4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57875 |
| Swiss Prot | Q9Z1P8 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | Arp4; Bk89; Fiaf; Ng27; Pgar; Hfarp; Pgarg; Pp1158; Angiopoietin-related protein 4; 425O18-1; Angiopoietin-like protein 4; Fasting-induced adipose factor; Hepatic fibrinogen/angiopoietin-related protein; Secreted protein Bk89; Angptl4; Farp |
| Background | Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity (By similarity). In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Angiopoietin-like 4 Polyclonal Antibody, Cy3 Conjugated (bs-5027R-Cy3) validated for, and at what dilution?
bs-5027R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Angiopoietin-like 4 Polyclonal Antibody, Cy3 Conjugated (bs-5027R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Rabbit.
How should bs-5027R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Angiopoietin-like 4 Polyclonal Antibody, Cy3 Conjugated (bs-5027R-Cy3)?
KLH conjugated synthetic peptide derived from mouse ARP4
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5027R-Cy3)?
Cy3 on bs-5027R-Cy3: excitation / emission 512,550nm/570,615nm.