Help

SAHH Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-5029R-BF750
Product Name SAHH Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SAHH
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 191
Swiss Prot P23526
Subcellular location Cytoplasm
Synonyms SAHH; adoHcyase; Adenosylhomocysteinase; S-adenosyl-L-homocysteine hydrolase; AHCY
Background Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SAHH Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-5029R-BF750) validated for, and at what dilution?

bs-5029R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SAHH Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-5029R-BF750) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-5029R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SAHH Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-5029R-BF750)?

KLH conjugated synthetic peptide derived from human SAHH

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-5029R-BF750)?

AbBy Fluor-750 on bs-5029R-BF750: excitation / emission 749nm/775nm.