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APOA2 Polyclonal Antibody

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
Overview
Catalog # bs-5034R
Product Name APOA2 Polyclonal Antibody
Applications
ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human APOA2
Immunogen Range 24-100/100
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 336
Swiss Prot P02652
Subcellular location Secreted
Synonyms apoAII; Apo-AII; ApoA-II; Apolipoprotein A-II; Apolipoprotein A2; APOA2
Background Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles. The apolipoprotein A II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Familial apolipoprotein A II deficiency may result from a splice junction alteration which blocks splicing of intron 3 from the primary transcript and result in the formation of a non functional mRNA.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is APOA2 Polyclonal Antibody (bs-5034R) validated for, and at what dilution?

bs-5034R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does APOA2 Polyclonal Antibody (bs-5034R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog.

How should bs-5034R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise APOA2 Polyclonal Antibody (bs-5034R)?

KLH conjugated synthetic peptide derived from human APOA2 Immunogen range: 24-100/100.