APOA2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5034R-Cy5 |
| Product Name | APOA2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human APOA2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 336 |
| Swiss Prot | P02652 |
| Subcellular location | Secreted |
| Synonyms | apoAII; Apo-AII; ApoA-II; Apolipoprotein A-II; Apolipoprotein A2; APOA2 |
| Background | Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles. The apolipoprotein A II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Familial apolipoprotein A II deficiency may result from a splice junction alteration which blocks splicing of intron 3 from the primary transcript and result in the formation of a non functional mRNA. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is APOA2 Polyclonal Antibody, Cy5 Conjugated (bs-5034R-Cy5) validated for, and at what dilution?
bs-5034R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APOA2 Polyclonal Antibody, Cy5 Conjugated (bs-5034R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog.
How should bs-5034R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APOA2 Polyclonal Antibody, Cy5 Conjugated (bs-5034R-Cy5)?
KLH conjugated synthetic peptide derived from human APOA2
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5034R-Cy5)?
Cy5 on bs-5034R-Cy5: excitation / emission 625,650nm/670nm.