Apolipoprotein E3 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5039R-APC |
| Product Name | Apolipoprotein E3 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody may detect APOE2, APOE3, and APOE4 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human APOE3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 348 |
| Swiss Prot | P02649 |
| Subcellular location | Secreted |
| Synonyms | AD2; LPG; APO-E; LDLCQ5; Apolipoprotein E; APOE |
| Background | Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Apolipoprotein E3 Polyclonal Antibody, APC Conjugated (bs-5039R-APC) validated for, and at what dilution?
bs-5039R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Apolipoprotein E3 Polyclonal Antibody, APC Conjugated (bs-5039R-APC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig.
How should bs-5039R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Apolipoprotein E3 Polyclonal Antibody, APC Conjugated (bs-5039R-APC)?
KLH conjugated synthetic peptide derived from human APOE3
What are the excitation and emission wavelengths of the APC conjugate (bs-5039R-APC)?
APC on bs-5039R-APC: excitation / emission 650nm/660nm.