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BDH1 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Horse
  • Rabbit
Overview
Catalog # bs-5041R-PerCP-100uL
Product Name BDH1 Polyclonal Antibody, PerCP Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Horse, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BDH1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 622
Subcellular location Cytoplasm
Synonyms 3 hydroxybutyrate dehydrogenase heart, mitochondrial; BDH; BDH-1; D beta hydroxybutyrate dehydrogenase, mitochondrial; 3-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; mitochondrial; BDH_HUMAN .
Background This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is BDH1 Polyclonal Antibody, PerCP Conjugated (bs-5041R-PerCP) validated for, and at what dilution?

bs-5041R-PerCP is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BDH1 Polyclonal Antibody, PerCP Conjugated (bs-5041R-PerCP) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Horse, Rabbit.

How should bs-5041R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BDH1 Polyclonal Antibody, PerCP Conjugated (bs-5041R-PerCP)?

KLH conjugated synthetic peptide derived from human BDH1

What are the excitation and emission wavelengths of the PerCP conjugate (bs-5041R-PerCP)?

PerCP on bs-5041R-PerCP: excitation / emission 482nm/677nm.

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