CYP39A1 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5050R-BF647-100uL |
| Product Name | CYP39A1 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CYP39A1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51302 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 24 hydroxycholesterol 7 alpha hydroxylase; cytochrome P450 family 39, subfamily A polypeptide 1; cytochrome P450 subfamily XXXIX oxysterol 7 alpha hydroxylase polypeptide 1 ; hCYP39A1; Oxysterol 7 alpha hydroxylase; oxysterol 7alpha hydroxylase; CP39A_HUMAN. |
| Background | The CYP39A1 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of steroids, cholesterol and other lipids. This endoplasmic reticulum protein is involved in the conversion of cholesterol to bile acids. Its substrates include the oxysterols 25-hydroxycholesterol, 27-hydroxycholesterol and 24-hydroxycholesterol. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CYP39A1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-5050R-BF647) validated for, and at what dilution?
bs-5050R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5050R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYP39A1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-5050R-BF647)?
KLH conjugated synthetic peptide derived from human CYP39A1
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-5050R-BF647)?
AbBy Fluor-647 on bs-5050R-BF647: excitation / emission 650nm/665nm.