CYP4A22 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5055R-Cy5-100uL |
| Product Name | CYP4A22 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CYP4A22 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2845411579 |
| Swiss Prot | Q02928, Q5TCH4 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CYP4A22; CYPIVA22; Cytochrome P450 4A22; Cytochrome P450 4A22K; Cytochrome P450 family 4 subfamily A polypeptide 22; Fatty acid omega hydroxylase; Lauric acid omega hydroxylase; RP1 18D14.1; CP4AM_HUMAN. |
| Background | Cytochrome P450 4A22 (CYP4A22) is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 1p33. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CYP4A22 Polyclonal Antibody, Cy5 Conjugated (bs-5055R-Cy5) validated for, and at what dilution?
bs-5055R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP4A22 Polyclonal Antibody, Cy5 Conjugated (bs-5055R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Horse, Rabbit.
How should bs-5055R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYP4A22 Polyclonal Antibody, Cy5 Conjugated (bs-5055R-Cy5)?
KLH conjugated synthetic peptide derived from human CYP4A22
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-5055R-Cy5)?
Cy5 on bs-5055R-Cy5: excitation / emission 625,650nm/670nm.