IDI1 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5073R-FITC |
| Product Name | IDI1 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IDI1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3422 |
| Swiss Prot | Q13907 |
| Subcellular location | Cytoplasm |
| Synonyms | IDI1; IPP isomerase 1; IPPI1; Isopentenyl diphosphate Delta isomerase 1; isopentenyl diphosphate dimethylallyl diphosphate isomerase 1; Isopentenyl pyrophosphate isomerase 1; IDI1_HUMAN. |
| Background | IDI1 is a peroxisomally localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP). These are substrates for the reactions that ultimately result in the synthesis of cholesterol. There is reduction in IPP isomerase activity in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IDI1 Polyclonal Antibody, FITC Conjugated (bs-5073R-FITC) validated for, and at what dilution?
bs-5073R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IDI1 Polyclonal Antibody, FITC Conjugated (bs-5073R-FITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Pig, Rabbit.
How should bs-5073R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IDI1 Polyclonal Antibody, FITC Conjugated (bs-5073R-FITC)?
KLH conjugated synthetic peptide derived from human IDI1
What are the excitation and emission wavelengths of the FITC conjugate (bs-5073R-FITC)?
FITC on bs-5073R-FITC: excitation / emission 494nm/518nm.