Ceramide synthase 2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5077R-Cy5.5-100uL |
| Product Name | Ceramide synthase 2 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Ceramide synthase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29956 |
| Swiss Prot | Q96G23 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | L3; LASS2; SP260; TMSG1; Ceramide synthase 2; CerS2; LAG1 longevity assurance homolog 2; Tumor metastasis-suppressor gene 1 protein |
| Background | LASS2 (LAG1 homolog, ceramide synthase 2) has sequence similarity to yeast longevity assurance gene 1. It is thought to be involved in sphingolipid synthesis. Expression of LASS2 is transiently increased during the period of active myelination and is specifically localized to white matter tracts of the brain, including the Schwann cells of sciatic nerves suggesting that LASS2 is important for the synthesis of dihydroceramide used for synthesis of myelin sphingolipids. Expression of LASS2 in hepatocellular carcinoma cell lines suppresses the growth of cancer cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Ceramide synthase 2 Polyclonal Antibody, Cy5.5 Conjugated (BS-5077R-CY5-5) validated for, and at what dilution?
BS-5077R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Ceramide synthase 2 Polyclonal Antibody, Cy5.5 Conjugated (BS-5077R-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Horse, Chicken, Rabbit.
How should BS-5077R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Ceramide synthase 2 Polyclonal Antibody, Cy5.5 Conjugated (BS-5077R-CY5-5)?
KLH conjugated synthetic peptide derived from human Ceramide synthase 2
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5077R-CY5-5)?
Cy5.5 on BS-5077R-CY5-5: excitation / emission 675nm/694nm.