Lamin B Receptor Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5081R-Cy3 |
| Product Name | Lamin B Receptor Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
|
| Specificity | The immunogen for this product has partial sequence similarity to SEPT8 and SEPT2 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Lamin B Receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3930 |
| Swiss Prot | Q14739 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | PHA; LMN2R; TDRD18; DHCR14B; Lamin-B receptor; Integral nuclear envelope inner membrane protein; LBR |
| Background | Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization. Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. The cleavage of lamins results in nuclear disregulation and cell death. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Lamin B Receptor Polyclonal Antibody, Cy3 Conjugated (bs-5081R-Cy3) validated for, and at what dilution?
bs-5081R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Lamin B Receptor Polyclonal Antibody, Cy3 Conjugated (bs-5081R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Horse, Rabbit.
How should bs-5081R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Lamin B Receptor Polyclonal Antibody, Cy3 Conjugated (bs-5081R-Cy3)?
KLH conjugated synthetic peptide derived from human Lamin B Receptor
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5081R-Cy3)?
Cy3 on bs-5081R-Cy3: excitation / emission 512,550nm/570,615nm.