LASS5 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5082R-Cy7 |
| Product Name | LASS5 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Possible cross-reactivity with CERS6/LASS6 (85% homology with the immunogen sequence) |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LASS5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55890 |
| Swiss Prot | Q8N5B7 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | LAG1 homolog ceramide synthase 5; LAG1 longevity assurance homolog 5; MGC45411; Trh4; CerS5; FLJ25304; CERS5_HUMAN. |
| Background | Lass5, or LAG1 longevity assurance homolog 5, is thought to be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donnor ceramide) in a fumonisin B1-independent manner. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is LASS5 Polyclonal Antibody, Cy7 Conjugated (bs-5082R-Cy7) validated for, and at what dilution?
bs-5082R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LASS5 Polyclonal Antibody, Cy7 Conjugated (bs-5082R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Chicken.
How should bs-5082R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LASS5 Polyclonal Antibody, Cy7 Conjugated (bs-5082R-Cy7)?
KLH conjugated synthetic peptide derived from human LASS5
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-5082R-Cy7)?
Cy7 on bs-5082R-Cy7: excitation / emission 743nm/767nm.