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NNT Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Dog

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-5097R-PerCP
Product Name NNT Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Dog
Predicted Reactivity Mouse, Rat, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NNT
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23530
Swiss Prot Q13423
Subcellular location Cytoplasm, Cell membrane
Synonyms GCCD4; NAD(P) transhydrogenase, mitochondrial; Nicotinamide nucleotide transhydrogenase; Pyridine nucleotide transhydrogenase; NNT
Background The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NNT Polyclonal Antibody, PerCP Conjugated (bs-5097R-PerCP) validated for, and at what dilution?

bs-5097R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NNT Polyclonal Antibody, PerCP Conjugated (bs-5097R-PerCP) react with?

Tested reactivity: Human, Dog. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-5097R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NNT Polyclonal Antibody, PerCP Conjugated (bs-5097R-PerCP)?

KLH conjugated synthetic peptide derived from human NNT

What are the excitation and emission wavelengths of the PerCP conjugate (bs-5097R-PerCP)?

PerCP on bs-5097R-PerCP: excitation / emission 482nm/677nm.