5-hmC Monoclonal Antibody(mouse)
Applications
Reactivity
| Overview | |
| Catalog # | bs-51025R-50ug |
| Product Name | 5-hmC Monoclonal Antibody(mouse) |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Monoclonal antibody raised in mouse against human ORC2, using two recombinant proteins containing the N-terminal and C-terminal part of the protein, respectively |
| Clonality | Monoclonal |
| Clone # | #REF! |
| Isotype | IgG1 |
| Concentration | 0.6ug/ul |
| Purification | Protein G purified monoclonal antibody in PBS containing 0.05% azide. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 4999 |
| Swiss Prot | Q13416 |
| Synonyms | ORC2L, Origin recognition complex subunit 2, ORC2 |
| Background | Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is 5-hmC Monoclonal Antibody(mouse) (bs-51025R) validated for, and at what dilution?
bs-51025R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 5-hmC Monoclonal Antibody(mouse) (bs-51025R) react with?
Tested reactivity: Human.
How should bs-51025R be stored?
Store at -20C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 5-hmC Monoclonal Antibody(mouse) (bs-51025R)?
Monoclonal antibody raised in mouse against human ORC2, using two recombinant proteins containing the N-terminal and C-terminal part of the protein, respectively