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PRPS1L1 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-5105R-Cy3
Product Name PRPS1L1 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PRS3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 221823
Subcellular location Cytoplasm
Synonyms PRS3; PRS-III; PRS III; PRS 3; PRS-3 Multicatalytic endopeptidase complex subunit C5; 20S proteasome beta type subunit antibody Proteasome component C5; PRS3; PTS1; YBL0407; PRPS3_HUMAN; PRPS1L1; PRPSL; Ribose-phosphate pyrophosphokinase 3; Phosphoribosyl pyrophosphate synthase 1-like 1; PRPS1-like 1; Phosphoribosyl pyrophosphate synthase III.
Background PRE7 is a subunit of the Yeast proteasome. The yeast proteasome seems to be composed of 14 different subunits which form a highly ordered ring-shaped structure. The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PRPS1L1 Polyclonal Antibody, Cy3 Conjugated (bs-5105R-Cy3) validated for, and at what dilution?

bs-5105R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PRPS1L1 Polyclonal Antibody, Cy3 Conjugated (bs-5105R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken.

How should bs-5105R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PRPS1L1 Polyclonal Antibody, Cy3 Conjugated (bs-5105R-Cy3)?

KLH conjugated synthetic peptide derived from human PRS3

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5105R-Cy3)?

Cy3 on bs-5105R-Cy3: excitation / emission 512,550nm/570,615nm.