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HCLS1 Polyclonal Antibody

Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti HCLS1\/LckBP1 Polyclonal Antibody, Unconjugated (bs-5109R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 53kD. Observed band size: 53kD.
Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti HCLS1\/LckBP1 Polyclonal Antibody, Unconjugated (bs-5109R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 53kD. Observed band size: 53kD.
Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-HCLS1 Polyclonal Antibody, Unconjugated (bs-5109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-HCLS1 Polyclonal Antibody, Unconjugated (bs-5109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-5109R
Product Name HCLS1 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HCLS1
Immunogen Range 161-260/486
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3059
Swiss Prot P14317
Subcellular location Cytoplasm, Cell membrane
Synonyms HS1; p75; CTTNL; lckBP1; Hematopoietic lineage cell-specific protein; Hematopoietic cell-specific LYN substrate 1; HCLS1
Background Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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