HCLS1 Polyclonal Antibody

$Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti HCLS1\/LckBP1 Polyclonal Antibody, Unconjugated (bs-5109R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 53kD. Observed band size: 53kD.$

Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti HCLS1\/LckBP1 Polyclonal Antibody, Unconjugated (bs-5109R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 53kD. Observed band size: 53kD.

Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-HCLS1 Polyclonal Antibody, Unconjugated (bs-5109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Horse
Rabbit

Overview
Catalog #	bs-5109R
Product Name	HCLS1 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HCLS1
Immunogen Range	161-260/486
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3059
Swiss Prot	P14317
Subcellular location	Cytoplasm, Cell membrane
Synonyms	HS1; p75; CTTNL; lckBP1; Hematopoietic lineage cell-specific protein; Hematopoietic cell-specific LYN substrate 1; HCLS1
Background	Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200