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H3K36me3 Polyclonal Antibody

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using the H3K36me3 Polyclonal Antibody H3K36me3 (bs-51116R) at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk.
Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using the H3K36me3 Polyclonal Antibody H3K36me3 (bs-51116R) at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk.
A Dot Blot analysis was performed to test the cross reactivity of H3K36me3 Polyclonal Antibody(bs-51116R) with peptides containing other H3 and H4 modifications and unmodified sequence. 0.2-100 pmol of peptide containing histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The result demonstrates high specificity of the antibody for the modification of interest.
A Dot Blot analysis was performed to test the cross reactivity of H3K36me3 Polyclonal Antibody(bs-51116R) with peptides containing other H3 and H4 modifications and unmodified sequence. 0.2-100 pmol of peptide containing histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The result demonstrates high specificity of the antibody for the modification of interest.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • dot-blot

Reactivity

  • Human
  • Zebrafish
  • Drosophila
Overview
Catalog # bs-51116r-50ug
Product Name H3K36me3 Polyclonal Antibody
Applications
WB ELISA DOT-BLOT
Reactivity Human, Zebrafish, Drosophila
Specifications
Conjugation Unconjugated
Host Rabbit
Source Polyclonal antibody raised in rabbit against histone H3, trimethylated at lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide
Clonality Polyclonal
Isotype Not Determined
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 8350
Swiss Prot P68431
Background Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
dot-blot

Frequently asked questions

Which applications is H3K36me3 Polyclonal Antibody (bs-51116R) validated for, and at what dilution?

bs-51116R is validated for WB (1:300-5000); ELISA (1:500-1000); dot-blot. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does H3K36me3 Polyclonal Antibody (bs-51116R) react with?

Tested reactivity: Human, Zebrafish, Drosophila.

How should bs-51116R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise H3K36me3 Polyclonal Antibody (bs-51116R)?

Polyclonal antibody raised in rabbit against histone H3, trimethylated at lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide

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