p53 Polyclonal Antibody

$Nuclear extracts of HeLa cells (40 \u03bcg) were analysed by Western blot using p53 Polyclonal Antibody (bs-51127R) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. Followed by secondary antibody incubation.$

Nuclear extracts of HeLa cells (40 \u03bcg) were analysed by Western blot using p53 Polyclonal Antibody (bs-51127R) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. Followed by secondary antibody incubation.

Applications

WB
ELISA

Reactivity

Human

Overview
Catalog #	bs-51127R
Product Name	p53 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein
Clonality	Polyclonal
Clone #	#REF!
Isotype	Not determined
Concentration	2.5ug/ul
Purification	Affinity purification
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	7157
Swiss Prot	P04637
Synonyms	TP53, P53, TRP53, LSF1
Background	Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2
Application Dilution
WB	1:300-5000
ELISA	1:500-1000