Cytochrome b5 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5115R-Cy5.5-100uL |
| Product Name | Cytochrome b5 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cytochrome b5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1528 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CYB 5; CYB 5A; CYB5; CYB5_HUMAN; CYB5A; Cytochrome b 5; Cytochrome b5 microsomal; Cytochrome b5; Cytochrome b5 type A microsomal; Cytochrome b5 type A; MCB 5; MCB5; Microsomal cytochrome b5; Microsomal cytochrome b5 type A; Type 1 cyt b5; CYB5A. |
| Background | The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Cytochrome b5 Polyclonal Antibody, Cy5.5 Conjugated (BS-5115R-CY5-5) validated for, and at what dilution?
BS-5115R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cytochrome b5 Polyclonal Antibody, Cy5.5 Conjugated (BS-5115R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig.
How should BS-5115R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cytochrome b5 Polyclonal Antibody, Cy5.5 Conjugated (BS-5115R-CY5-5)?
KLH conjugated synthetic peptide derived from human Cytochrome b5
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5115R-CY5-5)?
Cy5.5 on BS-5115R-CY5-5: excitation / emission 675nm/694nm.