GTPBP3 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5120R-FITC |
| Product Name | GTPBP3 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Horse, Chicken |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MSS1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5701 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | 26S protease complex; mammalian suppressor of sgv-1 of yeast; MSS1; Nbla10058; protease 26S subunit 7; proteasome 26S ATPase subunit 2; Proteasome 26S subunit ATPase 2; proteasome 26S subunit ATPase 2; Protein MSS1; PSMC2; putative protein product of Nbla10058; S7 antibody; PRS7_HUMAN. |
| Background | Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes and the endoplasmic reticulum. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GTPBP3 Polyclonal Antibody, FITC Conjugated (bs-5120R-FITC) validated for, and at what dilution?
bs-5120R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GTPBP3 Polyclonal Antibody, FITC Conjugated (bs-5120R-FITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Horse, Chicken.
How should bs-5120R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GTPBP3 Polyclonal Antibody, FITC Conjugated (bs-5120R-FITC)?
KLH conjugated synthetic peptide derived from human MSS1
What are the excitation and emission wavelengths of the FITC conjugate (bs-5120R-FITC)?
FITC on bs-5120R-FITC: excitation / emission 494nm/518nm.