ERp29 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5121R-BF350-100uL |
| Product Name | ERp29 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ERp29 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10961 |
| Subcellular location | Cytoplasm |
| Synonyms | Chromosome 12 open reading frame 8; Endoplasmic reticulum lumenal protein ERp28; Endoplasmic reticulum protein 29; Endoplasmic reticulum protein 29 isoform 1; Endoplasmic reticulum protein ERp29; ERp28; ERp31; PDI-DB; C12orf8. |
| Background | This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ERp29 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-5121R-BF350) validated for, and at what dilution?
bs-5121R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ERp29 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-5121R-BF350) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig.
How should bs-5121R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ERp29 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-5121R-BF350)?
KLH conjugated synthetic peptide derived from human ERp29
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-5121R-BF350)?
AbBy Fluor-350 on bs-5121R-BF350: excitation / emission 346nm/442nm.